Dental plaque is no match for catalytic nanoparticles
Twice-daily rinses of FDA-approved nanoparticles broke apart oral biofilms and prevented tooth decay in a study led by School of Dental Medicine researchers
Treatment with a nanoparticle and hydrogen peroxide (right panel) left little in the way of bacteria (in blue) or the sticky biofilm matrix (in red), making the combination a potent force against dental plaque.
Combine a diet high in sugar with poor oral hygiene habits and dental cavities, or caries, will likely result. The sugar triggers the formation of an acidic biofilm, known as plaque, on the teeth, eroding the surface. Early childhood caries is a severe form of tooth decay that affects one in every four children in the United States and hundreds of millions more globally. It’s a particularly severe problem in underprivileged populations.
The nanoparticles break apart dental plaque through a unique pH-activated antibiofilm mechanism.
“It displays an intriguing enzyme-like property whereby the catalytic activity is dramatically enhanced at acidic pH but is ‘switched off’ at neutral pH conditions,” says Koo, professor in Penn Dental Medicine’s Department of Orthodontics and in the divisions of Pediatric Dentistry and Community Oral Health. “The nanoparticles act as a peroxidase, activating hydrogen peroxide, a commonly used antiseptic, to generate free radicals that potently dismantle and kill biofilms in pathological acidic conditions but not at physiological pH, thus providing a targeted effect.”
Because the caries-causing plaque is highly acidic, the new therapy is able to precisely target areas of the teeth harboring pathogenic biofilms without harming the surrounding oral tissues or microbiota.
The particular iron-containing nanoparticle used in the experiments, ferumoxytol, is already FDA-approved to treat iron-deficiency, a promising indication that a topical application of the same nanoparticle, used at several-hundred-fold lower concentration, would also be safe for human use.
Though some scientists have questioned whether coatings used on ferumoxytol and other nanoparticles used for medical applications would render them catalytically inert, Koo, Cormode, and colleagues demonstrated that they maintained peroxidase-like activity, activating hydrogen peroxide.
After testing the ferumoxytol-hydrogen peroxide combination on a tooth-enamel-like material, the team moved on to an experimental set-up that more closely replicated the conditions of the human mouth.
“We used plaque samples from caries-active subjects to reconstruct these highly pathogenic biofilms on real human tooth enamel,” says Koo. “This simulation showed that our treatment not only disrupts the biofilm but also prevents mineral destruction of the tooth’s surface. That offered very strong evidence that this could work in vivo.”
Further studies in a rodent model that closely mirrors the stages of caries development in humans showed that twice-a-day rinses of ferumoxytol and hydrogen peroxide greatly reduced the severity of caries on all of the surfaces of the teeth and also completely blocked the formation of cavities in the enamel.
As further evidence of the treatment’s targeted effect, the researchers found no significant change in the diversity of microbes in the mouth after therapy and found no signs of tissue damage.
“This therapy isn’t killing microorganisms indiscriminately,” Koo says, “but rather it is acting only where the pathological biofilm develops. Such a precise therapeutic approach can target the diseased sites without disrupting the ecological balance of the oral microbiota, which is critical for a healthy mouth, while also avoiding infection by opportunistic pathogens.”
Incorporating nanoparticles in a mouth rinse or toothpaste could be a cost-effective way to significantly improve their effectiveness, says Koo. Many of these products already contain hydrogen peroxide and would only require the addition of a small amount of relatively inexpensive nanoparticles. With evidence backing this approach in both an animal model and a human-like model of tooth decay, the research team is actively working to test its clinical efficacy.
The paper’s lead author, Yuan Liu, a doctor of science in dentistry student in Koo’s lab, was also recognized for this work as one of two winners of the American Association for Dental Research’s Hatton Competition Award earlier this year.
In addition to Koo, Liu, and Cormode, coauthors were Geelsu Hwang, Dongyeop Kim, Yue Huang, Aurea Simon-Soro, Hoi-In Jung, Zhi Ren, Yong Li, and Faizan Alawi, all of Penn Dental Medicine; Pratap Naha and Sarah Gubara of Cormode’s lab; and Domenick Zero and Anderson Hara of Indiana University’s School of Dentistry.
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