Sophomores Linda Wu and Nova Meng spent the summer studying coevolution among plants, mutualistic bacteria, and parasitic nematodes in Corlett Wood’s biology lab.
To study coevolution, the responsibilities of Nova Meng and Linda Wu included caring for plants in the Penn greenhouse. (Image: From July 2021, when masks were not required)
Coevolution is all around us. Think of the elongated blooms that perfectly accommodate a hummingbird’s slender mouth parts. But not all examples of species influencing one another’s evolutionary course accrue benefits to all parties. Tradeoffs are part of the game.
This summer, sophomores Linda Wu of Annandale, Virginia, and Nova Meng of Akron, Ohio, researched an coevolutionary scenario with benefits as well as costs for the species involved. Their work, supported by the Penn Undergraduate Research Mentoring Program (PURM) and conducted in the lab of biology professor Corlett Wood, has examined the relationship among plants in the genus Medicago, beneficial bacteria that dwell in their roots, and parasitic nematodes that try to steal the plants’ nutrients.
Focusing on parasitic nematodes that draw nutrients from plants, Wu, who is pursuing a dual degree between Wharton and the College, is testing whether the parasites are specialists or generalists in the types of plants they infect.
The Center for Undergraduate Research & Fellowships provides students in the PURM program awards of $4,500 during the 10-week summer research internship. Wu and Meng stayed busy through those weeks. Whether evaluating plants in a soybean field in Michigan or tending to hundreds—even thousands—of plants in the greenhouse at Penn, these aspiring researchers built a foundation for future scientific endeavors with hands-on practice.
“It’s been an amazing experience,” says Wu. “I’ve always been interested in genetics and evolution and have found parasitic relationships in particular really interesting. I like reading about weird parasites. This summer I’ve gotten to participate in lab meetings, read books about coevolution, and expand my knowledge about the topic.”
Mentored by Ph.D. student McCall Calvert, Wu spent the summer focused on the parasites in the Medicago model system the Wood lab uses. “I’m trying to see if those nematodes are specialists or generalists, if they’re locally adapted to their host plant or open to parasitizing on different species,” Wu says.
To do so, she’s grown pots and pots of plants in the Penn greenhouse, experimentally infecting Medicago plants as well as other species, such as carrot and daisy plants, with nematodes, to measure the degree to which the parasites flourish.
Meng’s project looked at the bacterial side of the coevolutionary relationship. Overseen by lab manager and technician Eunnuri Yi, Meng looked at four strains of bacteria, known as rhizobia. Two strains are nitrogen-fixing, giving their associated plants a crucial nutrient to promote growth, while the other two do not seem to contribute nitrogen to the plants, and instead exist as parasites in the plants’ roots. “I’m looking at what happens when we infect the plants with nematode parasites,” Meng says, “to see if the plants that are open to mutualistic rhizobia are more susceptible to the nematode parasites.”
Meng, who is pursuing an engineering major, is examining how bacteria that dwell in plant roots affect the plants’ susceptibility to parasites.
While much of the students’ research duties took place in the lab or the greenhouse, Wu and Calvert had the opportunity to do field work as well. Earlier this month, they traveled to a long-term ecological research site run by Michigan State University to collect data on parasitic nematode outbreaks from cultivated fields as well as those allowed to turn over to a more natural state.
That data is informing a side project to Calvert’s main dissertation focus, one he couldn’t have accomplished without Meng, Wu and other undergraduates in the lab.
“Working with undergraduates satisfies two of the main missions I see as central to being a scientist,” says Calvert. “One is it allows us to educate others and impart the knowledge we’ve gained. And, also, when we have more people in the lab, we can better reach the second mission of conducting solid research.”
Wu’s research experience included a field trip to Michigan, where she and mentor McCall Calvert looked for parasites growing in soybean fields at a long-term ecological research site. (Image: Courtesy of Linda Wu)
As her first real experience in a lab, the PURM internship has given Wu a solid step toward pursuing a career she hopes will center on conservation. “I’ve been trying to find a study path that would involve environmental policy or sustainability, and I realized I wanted more of a STEM background,” she says. “I’d always been interested in science; I just didn’t have the opportunity to delve into it deeply in high school.”
Meng, who is considering attending medical school, has had her eyes opened to the curiosities and intriguing questions that arise when considering biology with an evolutionary lens. “I’ve been starting to look into Ph.D./M.D. programs,” she says, “I’m really enjoying the research aspect.”
For both, the close of the summer doesn’t mark an end to their time in the lab; Wu and Meng plan to continue working in the lab in the fall. “For me this wasn’t just a summer experience to have and be done with,” Wu says. “It was a great learning opportunity that I’m excited to continue.”
And the Wood lab is eager to welcome them back.
“One of the best things about the PURM program,” says Yi, “is that, hopefully, it is a launching pad for freshmen and younger students to start doing research at a higher level. I feel like making that initial, serious investment in these students encourages them to develop into what they’re capable of. I’m excited that Linda and Nova are coming back in the fall.”
Linda Wu is a sophomore pursuing an uncoordinated dual degree in business, energy, environment, and sustainability in the Wharton School and in biology with a concentration in ecology and evolution in the College of Arts and Sciences at the University of Pennsylvania.
Nova Meng is a sophomore majoring in bioengineering in the School of Engineering and Applied Science at Penn..
Eunnuri Yi is a lab manager and technician in the School of Arts & Sciences’ Department of Biology at Penn.
McCall Calvert is a Ph.D. student in the School of Arts & Sciences’ Department of Biology at Penn.
Corlett Wood is an assistant professor of biology in the University of Pennsylvania School of Arts & Sciences.
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